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buffer g3  (TaKaRa)


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    Structured Review

    TaKaRa buffer g3
    Buffer G3, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nucleobond+buffer+set+iii/NucleoBond+Buffer+Set+III/pmc13183943-382-6-12
    Average 93 stars, based on 36 article reviews
    buffer g3 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Sequencing:

    Article Title: Plasmid-mediated acquisition and chromosomal integration of bla CTX-M-14 in a subclade of Escherichia coli ST131- H 30 clade C1.
    Article Snippet: Escherichia coli ST131 is a multidrug-resistant lineage associated with the global spread of extended-spectrum β-lactamase-producing organisms.. Particularly, ST131 clade C1 is the most predominant clade in Japan, harboring blaCTX-M-14 at a high frequency.. However, the process of resistance gene acquisition and spread remains unclear.

    Article Title: Transmission of global clones of NDM-producing Enterobacterales and interspecies spread of IncX3 plasmid harboring bla NDM-5 in Tokyo.
    Article Snippet: .. DNA extraction, library preparation, and two-dimensional sequencing for MinION sequencing were performed with NucleoBond AXG 20 column (Takara Bio, Shiga, Japan) combined with NucleoBond Buffer Set III (Takara Bio), Rapid Barcoding Kit SQK-RBK004 (Oxford Nanopore Technologies), and R9.4 flow cell (Oxford Nanopore Technologies), respectively. .. Hybrid de novo assembly using both MiSeq and MinION reads was conducted with Unicycler (version 0.5.0) to characterize the plasmids carrying blaNDM.

    High Molecular Weight:

    Article Title: Plasmid-mediated acquisition and chromosomal integration of bla CTX-M-14 in a subclade of Escherichia coli ST131- H 30 clade C1.
    Article Snippet: Escherichia coli ST131 is a multidrug-resistant lineage associated with the global spread of extended-spectrum β-lactamase-producing organisms.. Particularly, ST131 clade C1 is the most predominant clade in Japan, harboring blaCTX-M-14 at a high frequency.. However, the process of resistance gene acquisition and spread remains unclear.

    Cell Culture:

    Article Title: Plasmid-mediated acquisition and chromosomal integration of bla CTX-M-14 in a subclade of Escherichia coli ST131- H 30 clade C1.
    Article Snippet: Escherichia coli ST131 is a multidrug-resistant lineage associated with the global spread of extended-spectrum β-lactamase-producing organisms.. Particularly, ST131 clade C1 is the most predominant clade in Japan, harboring blaCTX-M-14 at a high frequency.. However, the process of resistance gene acquisition and spread remains unclear.

    Control:

    Article Title: LINE1 promotes nuclear compartmentalization to repress the 8-cell state in embryonic stem cells
    Article Snippet: Three biological replicates per sample were prepared for NAD-seq following the crosslinked method of nucleoli isolation described by Vertii et al. .In brief, after serial sonication with a Misonix XL-2000 ultrasonic cell disruptor (10s each burst at full power) and re-suspension in high then low magnesium buffers, cell nucleoli were released and monitored under the microscope. .. The nucleolar-fraction DNA (NF-DNA) and the control whole-cell DNA (WC-DNA) were extracted using NucleoBond AGX-20 columns (Takara # 740544) and NucleoBond® Buffer Set III (Takara # 740603), following the manufacturer’s instructions. ..

    Article Title: LINE1 and PRC2 control nucleolar organization and repression of the 8C state in human ESCs.
    Article Snippet: Article LINE1 and PRC2 control nucleolar organization and repression of the 8C state in human ESCs

    Purification:

    Article Title: Complete genome sequence of the ammonia-oxidizing archaeon Nitrosopumilus zosterae type strain NM25.
    Article Snippet: .. The nucleic acids were extracted using a NucleoBond Buffer Set III and an AXG 500 column (TaKaRa Bio, Shiga, Japan) and purified using an AMPure XP beads (Beckman Coulter, CA, USA). .. The quality and quantity of the DNA were checked using an Agilent HS Genomic DNA 50 kb Kit (Agilent Technologies, CA, USA) and a QuantiFluor dsDNA System (Promega, WI, USA), respectively.

    DNA Extraction:

    Article Title: Transmission of global clones of NDM-producing Enterobacterales and interspecies spread of IncX3 plasmid harboring bla NDM-5 in Tokyo.
    Article Snippet: .. DNA extraction, library preparation, and two-dimensional sequencing for MinION sequencing were performed with NucleoBond AXG 20 column (Takara Bio, Shiga, Japan) combined with NucleoBond Buffer Set III (Takara Bio), Rapid Barcoding Kit SQK-RBK004 (Oxford Nanopore Technologies), and R9.4 flow cell (Oxford Nanopore Technologies), respectively. .. Hybrid de novo assembly using both MiSeq and MinION reads was conducted with Unicycler (version 0.5.0) to characterize the plasmids carrying blaNDM.



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